Horodithioate modification. Collection Symposium Series, 12:135-139(2011), XVth Symposium on Chemistry of Nucleic Acid Compounds, Cesky Krumlov, Czeck Republic, June 5-10, 2011. 4. Malgorzata Sierant, Xianbin Yang and Barbara Nawrot. SiRNA analogs containing phosphorodithioate substitutions. Phosphorus, Sulfur, and Silicon. 188: 427-436 (2013). 5. Sherry Y. Wu, Xianbin Yang* Kshipra M. Gharpure, Hiroto Hatakeyama, Martin Egli, Michael H. McGuire, Archana S. Nagaraja, Takahito M. Miyake, Rajesha Rupaimoole, Chad V. Pecot, Morgan Taylor, Sunila Pradeep, Malgorzata Sierant, Cristian Rodriguez-Aguayo, Hyun J. Choi, Rebecca A. Previs, Guillermo N. ArmaizPena, Li Huang, Carlos Martinez, Tom Hassell, Cristina Ivan, Vasudha Sehgal, Richa Singhania, Hee-Dong Han, Chang Su, Ji Hoon Kim, Heather J. Dalton, Chandra Kowali, Khandan Keyomarsi, Nigel A.J. McMillan, Willem W. Overwijk, Jinsong Liu, Ju-Seog Lee, Keith A. Baggerly, Gabriel Lopez-Berestein, Prahlad T. Ram, Barbara Nawrot, and Anil K. Sood*. 2′-OMephosphorodithioate modified siRNAs show increased loading into the RISC complex and enhanced antitumour activity. Nat. Commun. 5(12), 34593462 (2014). 6. Pardeep Pallan, Xianbin Yang*, Malgorzata Sierant, Nuwan Abeydeera, Tom Hassell, Carlos Martinez, Magdalena Janicka, Barbara Nawrot and Martin Egli*. Crystal structure, stability and Ago2 affinity of phosphorodithioate-modified RNAs. RSC Adv. DOI: 10.1039/c4ra10986d. 4(110), 6490164904 (2014).
NEW PRODUCT PAC-2-AMINO-dA
INTRODUCTION As we have noted in the past, the simplest approach to the design of high affinity primers and probes would be to substitute A sites with 2-amino-A, since the 2-amino-A-T base pair is equivalent in strength to the G-T base pair (Figure 1). 2-Amino-A also destabilizes A-G wobble mismatches, thus increasing specificity. In a manner analogous to dA, 2-aminodA is very susceptible to depurination during the acidic deblocking step of DNA synthesis. From previous development work, we know that acyl protecting groups at N6 seem to favor depurination while formamidine protecting groups seem to suppress depurination during detritylation.1953133-47-5 medchemexpress In 1998, we introduced the 2-amino-dA monomer (1), which appears to solve all of the earlier problems: deprotection is fast and effective; and it is stabilized to depurination during synthesis.2059148-82-0 custom synthesis However, the highly nucleophilic character of this bis formamidine protected diaminopurine leads to nucleophilic attack on the C-3′ of the sugar moiety during oxidation with iodine, resulting in strand scission.PMID:25905263 This problem can be reduced by employing non aqueous oxidation conditions, as described in Glen Report 23.2, page 11. Nevertheless, we concluded that it was time to consider a 2-Amino-dA monomer with optimized protection. We defined the following criteria for the “ideal” 2-Amino-dA monomer: Stable during oligonucleotide synthesis Stable during oxidation (see above) Stable during detritylation (depurination) Labile towards final deprotection conditions (NH3, AMA, MeNH2) The nucleobase protecting groups should be cleavable under mild conditions, e.g., compatible with 2-thio-dT containing sequences.
potentially leading to depurination during detritylation. Consequently, a formamidine protecting group at the N6 position is clearly indicated and dibutylformamidine is a good choice at the N6 position since it suppresses depurination and is easily removed from that position during deprotection. A formamidine at the N2 position causes some strand scission.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
