enohypophysis, composed of acidophilic cells, basophilic cells, chromophobe cells and neurohypophysis with all the standard nerve cells including nerve fibers and pituicyte, have been also definitely present within the yak hypophysis, which was separated by a clear dividing line. In yak testicular tissues, spermatogenic tubules and mesenchymal cells were observed. The seminiferous tubules have been arranged densely, and there were flat, contractile myoid cells on the outdoors from the basement membrane of each and every tubule. The connective tissue in between adjacent tubules contained polygonal Leydig cells. From the basement membrane, the spermatogenic cells and spermatogonia at many developmental stages may be noticed. Far more sperm cells, late metamorphosis sperm cells and sperm have been observed on the abluminal surface. The epithelium with the epididymis wall of the yak is composed mainly of principal cells, basal cells and smooth muscle cells. There were three to four layers of structure that may be seen in each component on the epithelium. A sizable number of mature sperm were distributed around the lumen surface, with the principal cells are distributed inside a tall column and the smooth muscle cell nuclei have been extended spindle-shaped and surrounded the outer periphery of every single epididymal tube propria. Basal cells have been within a single layer, distributed in the bottom on the propria of each and every epididymal duct. The cilia in Animals 2021, 11, x FOR PEER Evaluation lumen on the caput as well as the corpus epididymis have been longer, though the cilia inside the lumen six of 16 the from the cauda epididymis had been shorter, having a clear structure.Figure 1. H E staining of HPG tissues ofof an adult male yak (4 years old). A, B, C, D, E and of H E staining of hypothalaFigure 1. H E staining of HPG tissues an adult male yak (four years old). (A ) H E staining F hypothalamus, hypophysis, testis, hypophysis, testis, caput epididymis, corpus epididymis and cauda epididymis tissues, respectively (one hundred. The left mus, caput epididymis, corpus epididymis and cauda epididymis tissues, respectively (100. The box inside the decrease box in the is really a H4 Receptor Inhibitor medchemexpress partial enlarged view (400. H, ERK5 Inhibitor web hematocyte; NC, neurogliocyte; A, adenohypophysis; N, neurohypophysis; corner reduced left corner is usually a partial enlarged view (400. H, hematocyte; NC, neurogliocyte; A, adenohypophysis; N, neurohypophysis; P, pituicyte; cells; BC, basophilic BC, CC, chromophobe chromophobe fibers; ST, seminiferous tubule; P, pituicyte; AC, acidophilicAC, acidophilic cells; cells;basophilic cells; CC,cells; NF, nervecells; NF, nerve fibers; ST, seminiferous tubule; LC, myoid cells; LC, interstitial cells; SC, PS, principal spermatocyte; SS, secondary spermatocyte; S, MC, myoid cells; MC, interstitial cells; SC, spermatogonium;spermatogonium; PS, major spermatocyte; SS, secondary spermatocyte; S, spermatozoon; DET, ductuli efferentes testis; B, basal cells; Computer, principal cells; SM, smooth muscle cells; spermatozoon; DET, ductuli efferentes testis; B, basal cells; Pc, principal cells; SM, smooth muscle cells; CT, connective CT, connective tissue; DE, ductus epididymidis. tissue; DE, ductus epididymidis.3.three. Localization of NR1D1 and NR4A2 in Adult Yak Testis and Epididymis Tissues The IF signals of NR1D1, NR4A2 and 3-HSD proteins had been present in distinctive cell sorts in adult yak testis and epididymis tissues, specifically in Leydig cells (Figure 3 and Supplementary Components Figure S1). 3-HSD, as a precise molecular marker of Leydig cells, was present inside the cytoplasm of Leydig cells and where the NR1D1
