Ision-induced dissociation on species with an intensity threshold of 5,000 and charge
Ision-induced dissociation on species with an intensity threshold of 5,000 and charge states two and above. Data-dependent MS/MS have been acquired in centroid mode inside the ion trap utilizing 1 microscan, AGC target of 2E4, complete max IT of one hundred ms, 2.0 m/z isolation window, and normalized collision power of 35. DynamicSupplemental dataThe following supplies are obtainable in the on-line version of this article. Supplemental Data Set S1. Identification of differentially methylated regions in miP1a-OX versus Col-0 WT plants. Supplementary Data Set S2. List of SNPs present in miP1a-OX sum1 mutant plants, identified by complete genome sequencing. Supplementary Information Set S3. Identification of miP1a and miP1b interacting proteins in comparison to proteins immunoprecipitated from WT and 35S::FLAG-GFP transgenic plants. Supplementary Information Set S4. Identification of TPL and JMJ14 interacting proteins in comparison to proteins immunoprecipitated from WT and 35S::FLAG-GFP transgenic plants. Supplementary Figure S1. Expression levels on the miP1a transgene in prospective suppressor mutants. Supplementary Figure S2. The sum1 mutation could be the phenotype-causing mutation. Supplementary Figure S3. Flowering time analysis in short days. Supplementary Figure S4. CRISPR/Cas9 mediated targeted gene knockout of miP1a and miP1b. Supplementary Figure S5. Flowering time analysis of miP1a miP1b mutants in different photoperiods.AcknowledgmentsWe thank George Coupland, Christian Hardtke and Lars tergaard for offering seeds and Angiotensin-converting Enzyme (ACE) Inhibitor manufacturer Sebastian Marquardt for comments around the manuscript. We’re grateful towards the Yale proteomics center and the Quantitative Biology Center (QBiC) and Proteom Centrum Tubingen (PCT) at the Plant Physiology, 2021, Vol. 187, No.PLANT PHYSIOLOGY 2021: 187; 187|University of Tubingen, here the help of Mirita FranzWachtel and Boris Maek is specially acknowledged, for proc teomics analysis.FundingThis function was funded grants in the Deutsche Forschungsgemeinschaft (WE4281/7-1), the European Investigation Council (no. 336295), the Independent Study Fund Denmark (6108-00091, 0136-00015B and 0135-00014B), the Novo Nordisk Foundation (NNF18 OC0034226 and NNF19OC005658, and NNF20O C0061440) and start-up funding from the University of Copenhagen for the Copenhagen Plant Science Centre. Conflict of interest statement. None declared.
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