Morphology of fibroblasts was studied on the scaffolds after 7 days of
Morphology of fibroblasts was studied on the scaffolds after 7 days of culturing. SEM pictures indicated fibroblast cells formed standard spindle-shaped cells on all scaffolds (Fig 3A, B). As shown H E images of IL-4 Protein Gene ID scaffold devoid of cell (Fig 3C, D) and fibroblast cells were capable to penetrate, attach and grow in to the 3D structures of 3D spongy AM scaffold (Fig 3E, F) due to the presence of large pores. Cell metabolic activities in scaffolds Cell metabolic activity of fetal fibroblast cells in 3D spongy AM scaffolds were evaluated at each indicated time interval based MTS assay (Fig 3G).The outcomes of metabolic activity of human fetal fibroblast cells in 3D spongy AM scaffolds showed an rising trend over 7, 14, and 21 days, but no substantial differences had been observed through three and 7 days of incubation.CELL JOURNAL(Yakhteh), Vol 16, No 4, WinterFabrication of Spongy Denude AM ScaffoldABCDEFGFig 2: 3D AM scaffold using Russell- Movat staining (collagen, yellow) and (GAG, Green) (A). Cross linked ECM derived AM scaffold produced by freeze dryer (B). SEM image on the surface (C). The cross section with the porous (D). PBS swelling ratio of ECM derived human AM scaffolds at diverse times (E). In vitro collagenase biodegradation; time course of weight remaining of ECM derived HAM scaffold, cross-linked with ratio (1:4) of NHSEDC, after incubation in PBS containing 100 collagenase I, at 37 (F). Comparison results of impact of extract cytotoxicity of TCPs and scaffold groups on viability fetal fibroblast cells by MTS assay extract showed, (p0.05) (G). (Information are shown as mean normal deviation). ECM; Extracellular matrix, AM; Amniotic membrane, GAG; Lumican/LUM Protein Biological Activity Glycosaminoglycan, SEM; Scanning electronic microscopy, EDC; 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride, NHS; N-hydroxysuccinimide, PBS; Phosphate-buffered saline, TCP; Tissue culture plates, n=5, A; P0.001 and C; P0.05.CELL JOURNAL(Yakhteh), Vol 16, No four, WinterTaghiabadi et al.ABCDEFGFig three: SEM images of fetal fibroblast cells attached (arrows are indicating fibroblast cells) to ECM derived HAM scaffolds, after 7 days at surface (A) and internal surfaces of 3D spongy scaffold (B) obtained by cross sectioning. H E images ahead of and right after seeding cells, The light microscopy photos of H E pictures showed the external surface of scaffold with no cell (C) and attachment of human fetal fibroblast cells at external surfaces of scaffold, the arrows are indicating attachment of fetal fibroblast cells, the cells are dark grey and the AM scaffolds are light red (D). H E photos show the internal surface in the scaffold with out cell (E) attachment and development of fetal fibroblast cells at internal surface of scaffold following 7 days (F). MTS outcomes showed the metabolic activities of fetal fibroblast cells in ECM derived HAM scaffold. Statistical differences in metabolic activity at days 7, 14 and 21 with 3D HAM scaffold in days 3 (G). SEM; Scanning electronic microscopy, ECM; Extracellular matrix, HAM; Human amniotic membrane, H E; Hematoxylin and eosin. (Information are shown as imply regular deviation (SD). (n=5, A; P0.001 and B; P0. 01).CELL JOURNAL(Yakhteh), Vol 16, No four, WinterFabrication of Spongy Denude AM ScaffoldDiscussionAM is applied in surgery specifically for the reconstruction of traumatic wounds and skin transplantation (12). HAM is an proper substitute for general skin for surgical use resulting from its availability, low cost, and low danger of viral disease transmission and immunologic.
