Product Name: Lck (phospho Tyr505) antibody
Applications: WB
Predicted Target Size: 56 (note)
Positive Controls:
Form Supplied: Liquid
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Full Name: lymphocyte-specific protein tyrosine kinase
Background: Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR.
Synonyms: LSK, YT16, p56lck, pp58lck, LCK, t cell-specific protein-tyrosine kinase, tyrosine-protein kinase Lck, p56(LSTRA) protein-tyrosine kinase, proto-oncogene tyrosine-protein kinase LCK, leukocyte C-terminal Src kinase, T-lymphocyte specific protein tyrosine kinase p56lck, lymphocyte cell-specific protein-tyrosine kinase, lymphocyte-specific protein tyrosine kinase
Cellular Localization:
CAS NO: 53-86-1
Product: NLG919
Host: Rabbit
Clonality: Polyclonal
Isotype:
Immunogen: Peptide sequence around phosphorylation site of tyrosine 505 (G-Q-Y(p)-Q-P) derived from Human Lck.
Antigen Species: Human
Species Reactivity: Human, Mouse
Conjugation: Unconjugated
Storage Buffer: saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction: Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity:
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/20826815?dopt=Abstract
